During a stopover in Germany in the middle of a carefree road trip through Europe, two American girls find themselves alone at night when their car breaks down in the woods. Searching for help at a nearby villa, they are wooed into the clutches of a deranged retired surgeon who explains his mad scientific vision to his captives' utter horror. They are to be the subjects of his sick lifetime fantasy: to be the first to connect people, one to the next, and in doing so bring to life the human centipede. A mad scientist kidnaps and mutilates a trio of tourists in order to reassemble them into a human centipede, created by stitching their mouths to each others' rectums.